🧬Experiments

We have unlocked the first milestone - Fat Mouse.

This controlled experiment is designed to assess the effects of semaglutide on metabolic parameters in a high-fat diet-induced obesity model (Table 1.3). Male C57BL/6 mice, aged 8 weeks, are divided into two groups: a High-Fat group and a Control group, each consisting of 8 animals. The High-Fat group is fed a diet with 60% fat, 20% protein, and 20% carbohydrates and receives subcutaneous semaglutide injections (10 nmol/kg). The Control group, in contrast, is maintained on a 20% fat diet and treated with PBS injections. Both groups are subjected to their respective diets and treatments for 12 weeks to enable a clear comparison of the therapeutic effects of semaglutide versus the control condition.

Table 1.3: Details of the two experimental groups, including diet composition, treatment regimens, and 12-week intervention protocol.

The measurement regimen (Table 1.4) includes daily monitoring of body weight and food intake to track short-term and cumulative effects. Additionally, voluntary wheel running and body composition analyses are conducted every five days, providing insights into physical activity levels and body composition changes over the 12-week study. This combination of experimental design and frequent readouts ensures a robust framework for evaluating the impact of semaglutide on obesity-related outcomes.

Table 1.4: Overview of physiological and behavioral measurements, including frequency and duration, over the 12-week study period.